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. 2024 Jul 30;58(32):14249–14259. doi: 10.1021/acs.est.4c04545

Figure 5.

Figure 5

Efficiency of polyP extraction using plankton lysis buffer (plank. lysis) vs phenol-chloroform (phenol) from (A) wild-type C. reinhardtii collected using filtration, (B) wild-type C. reinhardtii collected using centrifugation, (C) V. perlucida collected using filtration, and (D) V. perlucida collected using centrifugation. The results are the amount of polyP (nanomoles) measured in 1 mL of culture samples of various cell quantities. The vertical bars represent the expected polyP amount in the samples (100% polyP), and the markers indicate results estimated using various extraction and quantification methods (e.g., plankton lysis-JC-D7 means polyP is extracted using the plankton lysis buffer and quantified using JC-D7 fluorescence).