Figure 2.

Schematic detailing the main concept of the Hoechst Pyronin Y staining protocol described within this paper

(A) MSC culture was set up and then co-cultured with PDX-ALL cells. PDX-ALL cells were then treated with drugs (if required) and incubated for the desired time. Cells were then harvested, filtered and stained, before flow cytometry analysis.

(B) images depicting various steps of the Hoechst Pyronin Y staining protocol. MSC-PDX-ALL co-cultures were set up and incubated for five days. 1 million cells were harvested from co-culture and passed through a 40 μM filter. PDX-ALL cells were centrifuged and resuspended in 1 mL of 10 μg/mL Hoechst. PDX-ALL cells were then incubated for 45 min. 5 μL of 100 μg/mL Pyronin Y was the added to Hoechst stained PDX-ALL cells before flow cytometry analysis.