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. 2024 Jul 20;5(3):103202. doi: 10.1016/j.xpro.2024.103202

Figure 3.

Figure 3

FCS Express analysis of CTV labeled PDX-ALL cells

(A) SSC-A versus FSC-A to gate intact cells.

(B) FSC-H versus FSC-A to gate single cells.

(C) FSC-A versus PI-A to gate live cells.

(D) FSC-A versus BV421-A to show proliferation with the cells to the far right indicating a retention of CTV labeling.

(E) A histogram used to show cell proliferation. Note: The results from this protocol were analyzed using FCS Express 7 software, however this can be done in any flow cytometry analysis software, the principle of each step will be the same.