FCS Express analysis of CTV labeled PDX-ALL cells
(A) SSC-A versus FSC-A to gate intact cells.
(B) FSC-H versus FSC-A to gate single cells.
(C) FSC-A versus PI-A to gate live cells.
(D) FSC-A versus BV421-A to show proliferation with the cells to the far right indicating a retention of CTV labeling.
(E) A histogram used to show cell proliferation. Note: The results from this protocol were analyzed using FCS Express 7 software, however this can be done in any flow cytometry analysis software, the principle of each step will be the same.