| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Biological samples | ||
| PDX-ALL, E2A/HLF | In-house1,3 | L707 |
| PDX-ALL, Biphenotypic/MLL rearrangement | In-house1,4 | MS40 |
| Human bone marrow mesenchymal stroma | In-house1,3 | 6255 |
| Chemicals, peptides, and recombinant proteins | ||
| Dexamethasone | Sigma-Aldrich, Dorset, UK | D4902 |
| Software and algorithms | ||
| FCSExpress Other softwares compatible with .FSC files such as FlowJo |
De Novo Software | Link to download: https://denovosoftware.com/full-access/download-landing/ |
| Other | ||
| BD FACSCanto II Machine or equivalent flow cytometer such as BD LSR, Cytek Aurora | BD Biosciences | |
| Low-glucose Dulbecco’s modified Eagle’s medium (DMEM) | Merck, Sigma-Aldrich | D5546 |
| FGF-basic recombinant human protein (10 mg) (bFGF) | BioLegend | 571506 |
| Vitronectin XF | STEMCELL Technologies, UK | Catalog #100-0763 |
| Serum-free medium for culture and expansion of hematopoietic cells (SFEM) | STEMCELL Technologies | 09655 |
| CellTrace Violet Cell Proliferation Kit | Thermo Fisher Scientific | Cat# C34557 |
| Propidium iodide (PI) | BD Biosciences | 51-66211E |
| Pyronin Y | Merck, Sigma-Aldrich | 213519-1G |
| Bisbenzimide H 33342 trihydrochloride (Hoechst) | Merck, Sigma-Aldrich | Cat# B2261 |
| Trypan blue solution | Gibco | 15250-061 |
| Fetal bovine serum (FBS) | Gibco | 10500-064 |
| Penicillin-Streptomycin solution | Gibco | 15140-122 |
| L-glutamine 200 mM (100×) | Gibco | 25030-024 |
| DMSO | Merck, Sigma-Aldrich | D2438-50ML |
| PBS | Gibco | 70011-044 |
| Trypsin-EDTA | Merck, Sigma-Aldrich | 15400054 |
| Pluri-strainer mini, 40 μM | PluriSelect | 43-10040-60 |
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