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| MSC medium | Final concentration (v/v) | Amount |
|---|---|---|
| Low-Glucose Dulbecco’s Modified Eagle Medium (DMEM) | 80% | 400 mL |
| Fetal Bovine Serum | 20% | 100 mL |
| Penicillin-Streptomycin | 0.1% | 5 mL |
| L-Glutamine | 0.1% | 5 mL |
| Total | 100% | 510 mL |
Storage conditions: Prepare Feeder cell medium under sterile conditions using a biosafety cabinet, store at 4°C and pre-warm to 37°C before use. Store at 4°C for up to 1 month.
