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| Patient-derived cell medium | Final concentration (v/v) | Amount |
|---|---|---|
| StemSpan SFEM Medium | 99.0% | 500 mL |
| Penicillin-Streptomycin | 1.0% | 5 mL |
| Total | 100% | 505 mL |
Storage conditions: Prepare patient-derived cell medium under sterile conditions using a biosafety cabinet, aliquot into 50 mL universal tubes and store at −20°C. Thaw aliquot to 37°C, do not refreeze and store at 4°C for up to 1 month.
