TABLE 5.
Sample methods included on NCBI’s public BioSample records
| Method | # of biosamples | # of labs | % of total |
|---|---|---|---|
| Sample type | |||
| Composite | 2,023 | 18 | 59.7 |
| Grab | 1,466 | 8 | 40.3 |
| Sample matrix | |||
| Raw wastewater | 3,306 | 19 | 97.5 |
| Post-grit removal | 79 | 1 | 2.3 |
| Primary effluent | 4 | 1 | 0.1 |
| Missing | 1 | 1 | <0.1 |
| Viral concentration method | |||
| Ceres Nanotrap | 1,212 | 8 | 35.8 |
| Innovaprep ultrafiltration | 604 | 5 | 17.8 |
| Promega wastewater large volume TNA capture kit | 455 | 5 | 13.4 |
| Peg precipitation + ultracentrifugation | 420 | 4 | 12.4 |
| Centricon 100k | 357 | 1 | 10.5 |
| Zymo water concentration buffer | 155 | 1 | 4.6 |
| Skim milk flocculation | 99 | 1 | 2.9 |
| Membrane filtration with acidification and MgCl2 | 64 | 2 | 1.9 |
| Innovaprep CP select | 19 | 1 | 0.5 |
| Backflushed raw ww using rexseed filters; Promega wastewater large column TNA capture kit | 4 | 1 | 0.1 |
| Nucleotide extraction method | |||
| Qiagen MagMAX Viral Kit | 939 | 2 | 27.7 |
| Promega Extraction Kits | 627 | 5 | 18.5 |
| Qiagen AllPrep Powerviral DNA/RNA kit | 424 | 4 | 12.5 |
| Qiagen Rneasy Powerwater Kit | 324 | 4 | 9.6 |
| Zymo quick-rna viral kit | 345 | 2 | 10.2 |
| Zymo Environ Water RNA Kit (R2042) | 262 | 1 | 7.7 |
| QIAamp Viral RNA mini kit | 201 | 5 | 5.9 |
| Neb monarch total rna miniprep kit + zymo onestep pcr inhibitor removal kit | 155 | 1 | 4.6 |
| Macherey-Nagel nucleomag DNA/RNA water kit | 108 | 1 | 3.2 |
| Ceres Nanotrap | 4 | 2 | 0.1 |
a
Methods cover type of sample collection, wastewater sample matrix, viral concentration method, and nucleotide extraction method.