Fig 4. MLH1-KQQ fails to promote polar resolution of sequence divergence at bases 1 and 2, but not 7 and 8, whereas MLH1-566-3A shows a defect at each base.

(A) Shown is the frequency of top strand base retention for WT, Mlh1^−/−, Mlh1^−/− + MLH1 (WT), Mlh1^−/− + KQQ mESCs. WT samples as in Fig 2B. n=3. *p ≤ 0.05, **p ≤ 0.005, *** ≤ 0.001. WT vs. Mlh1^−/−, unpaired t-test, Mlh1^−/− vs. Mlh1^−/− + MLH1 (WT) and Mlh1^−/− + KQQ, unpaired t-tests with Holm-Sidak correction. (B) Frequency of top strand base retention for WT, Mlh1^−/−, Mlh1^−/− + MLH1 (WT), Mlh1^−/− + 566–3A mESCs. WT samples as in Fig 2B. n=3. *p ≤ 0.05, **p ≤ 0.005, *** ≤ 0.001. WT vs. Mlh1^−/−, unpaired t-test, Mlh1^−/− vs. Mlh1^−/− + MLH1 (WT) and Mlh1^−/− + 566–3A, unpaired t-tests with Holm-Sidak correction. Data are represented as mean values ± SD.