Fig 5. MLH1-E34A, but not MLH1-RADA, suppresses divergent RMDs.

(A) Schematic diagram of MLH1 noting the location of E34A and RADA mutations. (B) Shown are the RMD frequencies in Mlh1^−/− mESCs transfected with EV, MLH1-WT, MLH1-E34A, or MLH1-RADA. Frequencies are normalized to transfection efficiency. n=6 for WT, n=9 for Mlh1^−/− + WT, E34A, and RADA. **p < 0.005, ns = not significant, unpaired t-tests. (C) Immunoblot show levels of MLH1-WT, E34A, and RADA. Data are represented as mean values ± SD.