Figure 1. Comparison of Eco1 retron to ssODN templated repair efficiency in a model genome editing assay.

(A) Schematic of Cas9-RT, msr-msd, and sgBFP expression vectors. (B) Experimental setup for transfection-based retron editing. HEK293T cells were transfected with Cas9-RT and ncRNA expressing plasmids and assessed for editing outcomes by flow cytometry 72h post-transfection. (C) Representative flow cytometry plots showing gates used to capture editing outcomes. Gates were drawn using an untransfected control. (D) Percentage of GFP positive cells by retron template source. In vitro transcribed RNA and GFP ssODN were transfected at two different concentrations, either 0.610pmols or 6.10pmols. Error bars denote standard error of the mean.