Figure 2. Genome editing efficiency of ncRNA architectures and delivery strategies.

(A) HDR editing outcomes from transfection-based editing assay as indicated by percentage of GFP+ cells. msr-msd-sgBFP (v2) represents an editor ncRNA with proposed improved structures. (B) Experimental setup for integrated ncRNA retron editing. Cells were transduced with ncRNA expressing lentivirus vectors and selected with puromycin 24h later. After one week of outgrowth, cells were transfected with plasmids expressing Cas9-RT and the corresponding ncRNA component in the case of integrated msr-msd or sgBFP. (C, D) NHEJ and HDR editing outcomes from an integrated ncRNA-based editing assay as indicated by percentage of BFP+ or GFP+ cells. Components that were expressed from a transfected plasmid are indicated (tfx), while all other ncRNA components were integrated into the genome by lentivirus. Error bars denote standard error of the mean.