Figure 2. PRMT5-pICln dependent methylation of SNRPB is required for chromatin accumulation.

a) Immunoblot of acid extracted chromatin over a time course of PRMT5 inhibition by GSK591. DMSO and MS023 controls are also present. b) Immunoblot of total cell extract of A549_dCas9-KRAB-MECP2 cells transduced with various sgRNAs. c) Immunoblot analysis of acid extracted chromatin of A549_dCas9-KRAB-MECP2 cells transduced with various sgRNAs. d) Immunoblot of IMR90-hTert cells over a time course of PRMT5 inhibition. e) PCA analysis of RNA-sequencing of PRMT5 and adaptor protein knockdowns. f) Comparison of ΔΨ (Delta PSI / Percent Spliced In) of retained introns (RI) following PRMT5 and adaptor protein knockdowns. g) Comparison of ΔΨ (Delta PSI) of skipped exons (SE) following PRMT5 and adaptor protein knockdowns.