Figure 9. Dynein targets Cen mRNA to centrosomes.

Maximum-intensity projections of NC 13 interphase embryos labeled with Cen smFISH (magenta), anti-Cnn antibodies (green; centrosomes), and DAPI (blue nuclei) in (A) WT, (B) Dhc^LOA hypomorphic, or (C) Khc^RNAi embryos. Quantification shows the percentage of total mRNA that (D) overlaps with centrosomes and (E) resides in granules at centrosomes (0 μm distance from Cnn). Each dot represents a measurement from N= 19 WT, 13 Dhc^LOA and 14 Khc^RNAi embryos. (E’) Log transformed RNA granule area from N=4127 granules from n=23 WT embryos and N=1412 granules from n=13 Dhc^LOA embryos; each dot represents a single granule. Mean ± SD displayed (red). Significance by (D and E) Kruskal-Wallis test followed by Dunn’s multiple comparison test relative to WT and (E’) unpaired t-test with n.s., not significant and ***, P<0.001. Scale bar: 5μm; 1 μm (inset).