Figure 3. The RGD motif in SNED1 is required to mediate cell adhesion.

(A) Schematic of SNED1 showing the two putative integrin-binding motifs in SNED1 (³⁸RGD and ³¹⁰LDV) and the point mutations introduced to disrupt their integrin-binding activity (³⁸RGD>RGE, ³¹⁰LDV>LAV).

(B, C) Adhesion of MDA-MB-231 ‘LM2’ breast cancer cells (B) and O9–1 mouse neural crest cells (C) to wild type or integrin-binding mutants (RGE, LAV or RGE/LAV) of SNED1. Data is represented as mean ± SD from three biological experiments. Welch and Brown-Forsythe one-way ANOVA with Dunnett’s T3 correction for multiple comparisons was performed to determine statistical significance. ns: non-significant, *p<0.05, ** p<0.01.