Figure 4. Functional blocking of integrins decreases cell adhesion to SNED1.

(A-B) Adhesion of MDA-MB-231 ‘LM2’ breast cancer cell (A) and O9–1 neural crest cells (B) to SNED1 is inhibited in presence of increasing concentrations of cRGDfV peptide. Data is represented as mean ± SD from three biological experiments. Welch and Brown-Forsythe one-way ANOVA with Dunnett’s T3 correction for multiple comparisons was performed to determine statistical significance. *p<0.05, **p<0.01.

(C) Schematic showing the seven integrin heterodimers previously predicted to interact with SNED1. Adapted from (Vallet et al., 2021).

(D) Immunoblots on total cell extract from MDA-MB-231 ‘LM2’ and O9–1 cells shows β1 integrin, α5 integrin, αv integrin and β3 integrins expression.

(E) Adhesion of MDA-MB-231 ‘LM2’ breast cancer cells to SNED1 is decreased in presence of anti-β1, anti-α5 and anti-αVβ3 integrin-blocking antibodies.

(F) Adhesion of O9–1 mouse neural crest cells to SNED1 is decreased in presence of anti-β1 and anti-α5 integrin blocking antibodies. Data is represented as mean ± SD from three biological experiments. Unpaired Student’s two-tailed t-test with Welch’s correction was performed to determine statistical significance. *p<0.05, ** p<0.01.