Figure 2:

Nociceptor-specific translating ribosome affinity purification (TRAP) sequencing after resolution of paclitaxel-induced mechanical hypersensitivity: A) Hierarchical clustering using the correlation coefficients showing separation between the IN and the IP samples. B) Heatmap plot revealing a clear distinction between the biological replicates between the TRAP-seq and the INPUT samples. C) Linear correlation plots show a higher correlation between the IP samples. D) Empirically probability distribution of all coding genes using the raw TPMs and quantile normalized TPMs of all IP samples. E) Higher expression is observed of neuronal genes (Mrgprd, Calcb, Scn10a, Calca, Trpv1, Prph) compared to non-neuronal genes (Prpdm12, Cx3cr1, Aif1, Mpz, Mbp, Gfap) showing neuron-specific enrichment using TRAP. F) Differentially translated mRNAs in the IP sample are shown by a dual-lightened plot against SSMD and log2-fold change.