Figure 5. Effect of psmf1 pan-neuronal knockdown on Drosophila melanogaster negative geotaxis measured by the climbing test.

Comparative climbing test (a) of three different Drosophila melanogaster RNA interference (RNAi) lines (prkn, psmf1, nsmase) with prkn used as positive control (significant motor impairment) and nsmase as a negative control (no motor impairment). (b) Climbing test performed at Days 10, 20 and 35 represented as percentage of flies that did not reach the 5-cm tube threshold. (c) Significant motor defect was observed at Day 35 for the psmf1 and prkn lines compared to the negative control nsmase. Significant comparisons are indicated by asterisks. ***p<0.0005. H. sapiens and D. melanogaster orthologues: PRKN (H. sapiens)/prkn (D. melanogaster); PSMF1 (H. sapiens)/psmf1 (D. melanogaster); SMPD2 (H. sapiens)/nsmase (D. melanogaster).