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. 2023 Dec 20;230(2):e254–e267. doi: 10.1093/infdis/jiad585

Table 1.

Primers and Probes Used for Quantitative Polymerase Chain Reaction to Detect Typhoidal Salmonella

Oligo Sequence (5′ to 3′) and Fluorophore Target Reference
oriC-probe FAM-TGATCTTCAGTGTTTCCCCAACCTGTTTTG-QSY Salmonella enterica (all serovars) [21]
oriC-F AGCCAAATCTCCGCTGGAT Salmonella enterica (all serovars) [21]
oriC-R CGGAACTGAAAGGCGCTG Salmonella enterica (all serovars) [21]
ST-probe VIC-CATTTGTTCTGGAGCAGGCTGACGG-QSY Salmonella Typhi staG (STY0201) [19]
ST-Frt CGCGAAGTCAGAGTCGACATAG Salmonella Typhi staG (STY0201) [19]
ST-Rrt AAGACCTCAACGCCGATCAC Salmonella Typhi staG (STY0201) [19]
PhHV-probe ABY-TTTTTATGTGTCCGCCACCATCTGGATC-QSY Recombinant pCR TOPO 2.1 gB [19]
PhHV-Frt GGGCGAATCACAGATTGAATC Recombinant pCR TOPO 2.1 gB [19]
PhHV-Rrt GCGGTTCCAAACGTACCAA Recombinant pCR TOPO 2.1 gB [19]

Abbreviations: PhHV, phocine herpesvirus.