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. 2024 Jul 4;27(8):110466. doi: 10.1016/j.isci.2024.110466

Figure 2.

Figure 2

Ca2+-induced conformational and spectral changes of Rec labeled with DyeA, DyeB, and DyeC

(A–C) The Ca2+-induced response of Rec with three BDI-derived labels (DyeA, DyeB, and DyeC). Blue curves correspond to samples in the presence of 100 μM CaCl2, and red curves correspond to calcium-free samples with 100 μM of chelator EGTA. The protein concentration was maintained at 10 μM. Excitation wavelengths were 440 nm, 460 nm, and 410 nm for DyeA, DyeB, and DyeC, respectively.

(D) Structural rearrangements of labeled Rec induced by calcium ions. The structures of the calcium-free and calcium-bound forms are based on PDB IDs 1IKU and 1JSA, respectively.51,53 DyeC attached to the single native cysteine (C39) in Rec is shown in yellow, and the myristoyl group at the N terminus of Rec is shown in black. Rec is colored in a gradient from red on its N terminus to cyan on the C terminus.