Table 2.
Anatomic Distribution of Ad5.UbP.GFP-transduced DO11.hCARΔcyt versus Control DO11.10 T Cell Populations following Adoptive Transfer
| % of Total KJ1-26+ Cells in Each Lymphoid Tissue Microenvironmenta | |||
| Tissue/Microenvironent | DO11.10 | GFP-positive DO11.hCARΔcyt | |
| Spleen | |||
| PALS | 92b | 91 | |
| Primary Nodules/Follicles | 3 | 5 | |
| Marginal Zone | 4 | 2 | |
| Red Pulp | 1 | 3 | |
| Inguinal LN | |||
| Diffuse Cortex/Paracortex | 98 | 97 | |
| Primary Nodules/Follicles | 2 | 3 | |
| Medullary Cords | 0 | 0 | |
| Mesenteric LN | |||
| Diffuse Cortex/Paracortex | 97 | 96 | |
| Primary Nodules/Follicles | 3 | 4 | |
| Medullary Cords | 0 | 0 | |
| Peyer's Patch | |||
| Paranodular zones (T cell) | 99 | 98 | |
| Nodules (B cell) | 1 | 2 | |
a Two days following transfer of 5 × 106 DO11.10 or Ad5.UbP.GFP-transduced DO11.hCARΔcyt CD4+ T cells into BALB/c recipients, animals were sacrificed and the recovered lymphoid tissues were processed for microscopic analysis as described in Material and Methods. b Numbers are the fraction of the total cell population in each tissue. Definition of the lymphoid tissue microenvironments follows the nomenclature of Weiss. Data are the mean from 3–4 animals per group (standard deviations <16%).