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. 2024 Aug 15;15:7041. doi: 10.1038/s41467-024-50808-w

Fig. 5. Sustained representation of noni odour stimuli in PNs of D. sechellia.

Fig. 5

a–d Whole-cell patch clamp recording from VM5d PNs in D. melanogaster and D. sechellia; glomerular circuitry is schematised on the left. Genotypes as in Fig. 4f. a Voltage trace of VM5d PNs in response to a pulse of 2-heptanone, and comparison of input resistance (b), resting membrane potential (c), and spontaneous activity (d) between species. b–d mean ± SEM are shown. n = 4 (D. melanogaster) and 5 (D. sechellia) animals. e Dose-response relationship of VM5d PN firing to 2-heptanone stimulation. Quantification of spike frequency was performed in a 50 ms window covering the peak response. Mean ± SEM are shown. n = 2–5 animals (Supplementary Table 1). EC50 values [Log] are as follows: −7.46 (Dmel), −6.87 (Dsec). f VM5d PN spike frequency in response to 10−6 dilution of 2-heptanone. Left, time course of spike frequency. Mean ± SEM are shown. Right, quantification of the decay magnitude (i.e., start (first 50 ms) - end (last 500 ms before odour offset)). n = 5 animals each. g, h Dose-dependent, odour-evoked calcium responses in Or85b/VM5d (g) and Or22a/DM2 (h) OSN axon termini and PN dendrites in the antennal lobe of D. melanogaster and D. sechellia, reported as normalised GCaMP6f fluorescence changes. Plots are based on the data in Supplementary Fig. 8. EC50 values [Log] are as follows: Or85b OSNs: −6.50 (Dmel), −6.51 (Dsec); VM5d PNs: –7.82 (Dmel), −7.87 (Dsec); Or22a OSNs: −7.42 (Dmel), −8.23 (Dsec); DM2 PNs: −7.97 (Dmel), −9.61 (Dsec). Genotypes are as follows. OSN imaging: D. melanogaster w;UAS-GCaMP6f/Orco-Gal4,UAS-GCaMP6f, D. sechellia w,UAS-GCaMP6f/w,UAS-GCaMP6f;;+/DsecOrcoGal4. PN imaging: D. melanogaster w;UAS-GCaMP6f/+;+/VT033008-Gal4 (VM5d PNs), D. melanogaster w;UAS-GCaMP6f/+;+/VT033006-Gal4 (DM2 PNs), D. sechellia w,UAS-GCaMP6f/w,VT033008-Gal4 (VM5d PNs), w,UAS-GCaMP6f/w,VT033006-Gal4 (DM2 PNs). i Responses of Or85b OSNs to pulsed odour stimuli (ten 200 ms pulses, each separated by 200 ms, as indicated by the black bars). For both species, left panels show the time course (mean ± SEM ΔF/F0) and right panels show the quantification of ΔF/F0 peak to the 1st and 10th stimulation. n = 8 animals each. Genotypes as in g. j, k Pulsed odour responses of VM5d PNs (j) and DM2 PNs (k). Mean ± SEM are shown in the time course. n = 8 animals each. Genotypes as in g, h. l, m Pulsed odour responses of DM4 (Or59b) and VA2 (Or92a) PNs (two control pathways where the number of partner OSNs is conserved between species (Fig. 1c)). Mean ± SEM are shown in the time course. n = 6 animals each. See Supplementary Fig. 11 for dose-response data. Genotypes are as for DM2 PN imaging in h. For a–f Student’s t test (two-sided) and for i–m paired t test (two-sided): ***P < 0.001, **P < 0.01, *P < 0.05, NS P > 0.05.