Table 1.
Free amino acid | Control | LP | LP + SC | LP + PK | LP + PK + SC |
---|---|---|---|---|---|
Aspartic acid | 1.35 ± 0.06c | 0.90 ± 0.04d | 0.52 ± 0.01e | 2.75 ± 0.05a | 1.97 ± 0.02b |
Glutamic acid | 4.08 ± 0.14d | 4.42 ± 0.15c | 4.93 ± 0.28b | 4.97 ± 0.64b | 5.51 ± 0.21a |
Serine | 0.48 ± 0.04a | 0.26 ± 0.02b | 0.43 ± 0.04ab | 0.31 ± 0.03ab | 0.37 ± 0.02ab |
Glycine | 0.35 ± 0.07ab | 0.36 ± 0.06ab | 0.28 ± 0.04b | 0.43 ± 0.08a | 0.42 ± 0.07a |
Histidine | 0.94 ± 0.22c | 1.72 ± 0.27ab | 1.41 ± 0.20bc | 2.15 ± 0.16a | 2.01 ± 0.27a |
Threonine | 1.21 ± 0.38b | 0.70 ± 0.12c | 1.87 ± 0.34a | 1.91 ± 0.14a | 1.78 ± 0.10a |
Alanine | 1.18 ± 0.09a | 0.89 ± 0.07b | 0.95 ± 0.22b | 1.10 ± 0.21a | 0.92 ± 0.15b |
Arginine | 3.46 ± 0.41a | 3.50 ± 0.35a | 3.79 ± 0.17a | 2.56 ± 0.29b | 2.37 ± 0.12b |
Proline | 0.34 ± 0.06b | 0.03 ± 0.02c | 0.90 ± 0.05a | 0.07 ± 0.03c | 0.04 ± 0.02c |
Tyrosine | 0.23 ± 0.06a | 0.19 ± 0.05ab | 0.18 ± 0.05ab | 0.22 ± 0.06a | 0.13 ± 0.04b |
Valine | 0.15 ± 0.02c | 0.24 ± 0.03b | 0.22 ± 0.02b | 0.30 ± 0.02a | 0.30 ± 0.01a |
Methionine | 0.15 ± 0.02a | 0.09 ± 0.03ab | 0.06 ± 0.04b | 0.17 ± 0.03a | 0.14 ± 0.04ab |
Cysteine | 1.45 ± 0.32a | 1.63 ± 0.28a | 1.95 ± 0.28a | 1.75 ± 0.30a | 1.62 ± 0.21a |
Isoleucine | 0.30 ± 0.05a | 0.28 ± 0.05a | 0.31 ± 0.06a | 0.31 ± 0.05a | 0.27 ± 0.04a |
Leucine | 0.38 ± 0.01c | 0.41 ± 0.03bc | 0.38 ± 0.03c | 0.53 ± 0.02a | 0.44 ± 0.06b |
Phenylalanine | 0.16 ± 0.01a | 0.07 ± 0.01b | 0.08 ± 0.02b | 0.08 ± 0.01b | 0.07 ± 0.01b |
Lysine | 1.13 ± 0.19a | 1.09 ± 0.11a | 1.03 ± 0.20a | 0.99 ± 0.10a | 1.00 ± 0.17a |
Total | 17.7 ± 0.64d | 17.0 ± 0.71d | 19.2 ± 0.54c | 21.6 ± 0.81a | 20.3 ± 0.66b |
a-d Means within the same row with different lowercase letters differ significantly (p < 0.05).
Control: noninoculation; LP: Lactiplantibacillus plantarum S8; LP + SC: Lactiplantibacillus plantarum S8 + Staphylococcus carnosus L8; LP + PK: Lactiplantibacillus plantarum S8 + Staphylococcus carnosus L8; LP + SC + PK: Lactiplantibacillus plantarum S8 + Staphylococcus carnosus L8 + Pichia kudriavzevii M6.