Figure 2.

Gene and protein expression analysis of calpain 1, 2, and a3 (CAPN1, CAPN2, and CAPN3), calpastatin (CAST) and calpain small subunits 1 and 2 (CAPNS1 and CAPNS2) on human brain microvascular endothelial cells (HBMECs), low-grade glioma-derived endothelial cells (LGG-ECs), and glioblastoma endothelial cells (GECs). (A) Gene expression obtained by quantitative real-time PCR and reported as fold change to HBMECs, used as control. (B) Representative images of Western blot for calpain signaling and relative densitometric analysis (C) obtained by Fiji ImageJ, to quantify protein expression level, normalized to GAPDH expression level, used as endogenous control, and to untreated CTRL. Data are the mean±SD of at least three independent experiments run in triplicate. *p<0.05, **p<0.01, ***p<0.001 vs. HBMECs.