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(A) MTT assay performed after the administration of different doses of calpain inhibitor 1–2 and calpastatin. (B) MTT assay performed on healthy brain cells, astrocytes, and neural progenitor stem cells (NPSC) to assess the possible toxic effect of calpain inhibitors. (C) Representative images of Live and Dead assay conducted to assess the toxicity of tested compounds. Data are the mean±SD of at least three independent experiments run in triplicate. **p<0.01, ***p<0.001.
