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Phylogenetic analyses of 16S rRNA gene (a) and identity (b) show the affiliations of these three new NOB bacteria (red font) and known NOB strains (black font), Maximum likelihood tree of 43 concatenated markers gene (c) show the affiliations of retained for NOB bacteria (red font) and known NOB strains (black font). 16S rRNA gene tree was rooted by 16S rRNA genes of Nitrotoga, 43 markers phylogenetic tree was rooted by midpoint.
