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. 1992 Jul 15;285(Pt 2):569–576. doi: 10.1042/bj2850569

An e.x.a.f.s. study of the manganese O2-evolving complex in purified Photosystem II membrane fractions. The S1 and S2 states.

D J MacLachlan 1, B J Hallahan 1, S V Ruffle 1, J H Nugent 1, M C Evans 1, R W Strange 1, S S Hasnain 1
PMCID: PMC1132826  PMID: 1637347

Abstract

Manganese K-edge X-ray spectra have been obtained for Photosystem II samples isolated using Triton X-100 detergent and samples further purified by n-heptyl beta-D-thioglucoside detergent treatment to remove light-harvesting polypeptides and low-affinity calcium. The structure of the manganese complex is very similar for the two detergent preparations used. Analysis of the e.x.a.f.s. spectra for samples in the S1 and S2 states indicate changes in bond lengths for the shells of oxygen/nitrogen atoms. For the S1 state, oxygen shells at 0.181 and 0.193 nm (1.81 and 1.93 A) were observed and one manganese shell at 0.270 nm (2.70A). In the S2 state the oxygen bond lengths are longer at 0.184 and 0.200 nm (1.84 and 2.00 A). Additionally a shell of scatterers at 0.37 nm (3.7 A) was observed in both states which could be fitted to models with calcium scatterers at this distance.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Boussac A., Zimmermann J. L., Rutherford A. W. EPR signals from modified charge accumulation states of the oxygen evolving enzyme in Ca2+-deficient photosystem II. Biochemistry. 1989 Nov 14;28(23):8984–8989. doi: 10.1021/bi00449a005. [DOI] [PubMed] [Google Scholar]
  2. Cheniae G. M., Martin I. F. Sites of function of manganese within photosystem II. Roles in O2 evolution and system II. Biochim Biophys Acta. 1970 Mar 3;197(2):219–239. doi: 10.1016/0005-2728(70)90033-2. [DOI] [PubMed] [Google Scholar]
  3. Cole J. L., Yachandra V. K., McDermott A. E., Guiles R. D., Britt R. D., Dexheimer S. L., Sauer K., Klein M. P. Structure of the manganese complex of photosystem II upon removal of the 33-kilodalton extrinsic protein: an X-ray absorption spectroscopy study. Biochemistry. 1987 Sep 22;26(19):5967–5973. doi: 10.1021/bi00393a004. [DOI] [PubMed] [Google Scholar]
  4. Cole J., Yachandra V. K., Guiles R. D., McDermott A. E., Britt R. D., Dexheimer S. L., Sauer K., Klein M. P. Assignment of the g = 4.1 EPR signal to manganese in the S2 state of the photosynthetic oxygen-evolving complex: an X-ray absorption edge spectroscopy study. Biochim Biophys Acta. 1987 Mar 4;890(3):395–398. doi: 10.1016/0005-2728(87)90169-1. [DOI] [PubMed] [Google Scholar]
  5. Dismukes G. C., Siderer Y. Intermediates of a polynuclear manganese center involved in photosynthetic oxidation of water. Proc Natl Acad Sci U S A. 1981 Jan;78(1):274–278. doi: 10.1073/pnas.78.1.274. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. George G. N., Prince R. C., Cramer S. P. The manganese site of the photosynthetic water-splitting enzyme. Science. 1989 Feb 10;243(4892):789–791. doi: 10.1126/science.2916124. [DOI] [PubMed] [Google Scholar]
  7. Guiles R. D., Yachandra V. K., McDermott A. E., Cole J. L., Dexheimer S. L., Britt R. D., Sauer K., Klein M. P. The S0 state of photosystem II induced by hydroxylamine: differences between the structure of the manganese complex in the S0 and S1 states determined by X-ray absorption spectroscopy. Biochemistry. 1990 Jan 16;29(2):486–496. doi: 10.1021/bi00454a024. [DOI] [PubMed] [Google Scholar]
  8. Guiles R. D., Zimmermann J. L., McDermott A. E., Yachandra V. K., Cole J. L., Dexheimer S. L., Britt R. D., Wieghardt K., Bossek U., Sauer K. The S3 state of photosystem II: differences between the structure of the manganese complex in the S2 and S3 states determined by X-ray absorption spectroscopy. Biochemistry. 1990 Jan 16;29(2):471–485. doi: 10.1021/bi00454a023. [DOI] [PubMed] [Google Scholar]
  9. Hardman K. D., Ainsworth C. F. Structure of concanavalin A at 2.4-A resolution. Biochemistry. 1972 Dec 19;11(26):4910–4919. doi: 10.1021/bi00776a006. [DOI] [PubMed] [Google Scholar]
  10. Kok B., Forbush B., McGloin M. Cooperation of charges in photosynthetic O2 evolution-I. A linear four step mechanism. Photochem Photobiol. 1970 Jun;11(6):457–475. doi: 10.1111/j.1751-1097.1970.tb06017.x. [DOI] [PubMed] [Google Scholar]
  11. McDermott A. E., Yachandra V. K., Guiles R. D., Cole J. L., Dexheimer S. L., Britt R. D., Sauer K., Klein M. P. Characterization of the manganese O2-evolving complex and the iron-quinone acceptor complex in photosystem II from a thermophilic cyanobacterium by electron paramagnetic resonance and X-ray absorption spectroscopy. Biochemistry. 1988 May 31;27(11):4021–4031. doi: 10.1021/bi00411a019. [DOI] [PubMed] [Google Scholar]
  12. Perutz M. F., Hasnain S. S., Duke P. J., Sessler J. L., Hahn J. E. Stereochemistry of iron in deoxyhaemoglobin. Nature. 1982 Feb 11;295(5849):535–538. doi: 10.1038/295535a0. [DOI] [PubMed] [Google Scholar]
  13. Rutherford A. W. Photosystem II, the water-splitting enzyme. Trends Biochem Sci. 1989 Jun;14(6):227–232. doi: 10.1016/0968-0004(89)90032-7. [DOI] [PubMed] [Google Scholar]
  14. Yachandra V. K., Guiles R. D., McDermott A. E., Cole J. L., Britt R. D., Dexheimer S. L., Sauer K., Klein M. P. Comparison of the structure of the manganese complex in the S1 and S2 states of the photosynthetic O2-evolving complex: an x-ray absorption spectroscopy study. Biochemistry. 1987 Sep 22;26(19):5974–5981. doi: 10.1021/bi00393a005. [DOI] [PubMed] [Google Scholar]

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