Figure 5.

Effect of long-term high-fat diet feeding non-alcoholic fatty liver and circadian rhythm gene expression in the liver of wild-type and G6PD^S188Frats.A, G6PD can regulate metabolic pathways. Prediction legends showing various symbols and arrows for interpretation of the IPA analysis are from Figure 2. B, representative images of H&E staining showing lipid accumulation and fatty liver in HFD-fed wild-type (WT) rats and to a lesser extent in G6PD^S188F rats. Images were reused to demonstrate rigor and reproducibility in Fig. S6. C, representative images of Oil Red staining indicate that lipid droplets are reduced in the liver of G6PD^S188F rats than in wild-type (WT) rats. D and E, expression of circadian clock gene Bmal1 decreased in the liver of both genotypes fed with HFD, while circadian clock and Prader-Willi Syndrome associated Magel2 gene expression selectively increased in HFD-fed G6PD^S188F rats. F, overexpression of MAGEL2 significantly reduced insulin-induced fatty acid uptake and accumulation in HepG2 cells. Two-way ANOVA with post hoc Tukey’s multiple comparison test was used to compare multiple groups. ∗∗p < 0.01 and ∗∗∗∗p < 0.001.