Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Aug 16;10(33):eado0424. doi: 10.1126/sciadv.ado0424

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2024 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY).

This is an open-access article distributed under the terms of the Creative Commons Attribution license, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Fig. 3. — (A) Nanos2 expression in male mesenteries. (B) Nanos2 expression in female mesenteries. Nanos2⁺ trophocytes are marked by an arrow. (C) Detailed picture of (B). (D to I) Nanos2::mOr expression in adult gonads. (D) Female gonad showing reporter-expressing trophonemata located closely to the germinal vesicle of each oocyte (arrowheads). (E) Nanos2::mOr-expressing trophonema (arrowhead). Nuc, nucleus of the oocyte. (F) Detailed picture of (E) (DAPI not displayed). (G) Male gonad, with mOr expressing in the gastrodermis around the spermaries and the bordering tissue of the ciliated tract. The ciliated plug is indicated by an arrowhead. (H) Detailed picture of a ciliated plug (arrowhead) in the gastrodermis surrounding the sperm chambers. Cells composing the plug can be identified by increased mOr expression and phalloidin incorporation (yellow). (I) Detailed picture of (H) (DAPI not displayed). (J) Piwi1::mOr expression in male mesenteries. (K) Piwi1::mOr expression in female mesenteries. (L) Detailed picture of the mOr⁺ maturing oocytes in (K). (M to R) Piwi1::mOr expression in adult gonads. (M) Female gonad. (N) Oocyte with the mOr⁻ trophonema indicated by an arrowhead. (O) Detailed picture of (N). (P) Male gonad expressing piwi1::mOr in the endomesoderm surrounding the sperm chambers. The ciliated plug is indicated by an arrowhead. (Q) A ciliated plug (arrowhead) in the gastrodermis surrounding the sperm chambers. Cells composing the plug can be identified by increased mOr expression and phalloidin incorporation (yellow). (R) Detailed picture of (Q) (DAPI not displayed). For all fluorescent pictures, mOr is displayed as magenta, DAPI is displayed as white, and phalloidin is displayed as yellow.