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. 2024 Jun 12;13(8):738–749. doi: 10.1093/stcltm/szae039

Figure 5.

Figure 5.

MSC-Ad.GS2 shows tumor tropism in vitro and in vivo. (A) MSCs exhibit tropism toward tumor-conditioned medium (TCM) as assessed using a 48-well chemotaxis chamber. TCM or MSC-conditioned medium (MCM) was placed in the lower chamber, whereas MSCs were added to the upper chamber. Migratory cells on the lower surface of the membrane were fixed, stained with Giemsa solution, and photographed after culture for 6 h (magnification × 40; scale bar = 200 μm; left panel). Migratory cells were counted in 3 high-power fields using the ImageJ software (right panel). Values shown are mean ± SD (n = 3). (B) Biodistribution analysis of MSC-Ad.GS2 and Ad.GS2 in MBT-2 tumor-bearing mice by in vivo bioluminescence imaging. C3H/HeN mice were inoculated intraperitoneally with 1.2 × 106 MBT-2 cells on day 0 and injected via the same route with 2 × 106 MSC-Ad.GS2 or 5 × 108 PFU of Ad.GS2 on day 11. Bioluminescent signals and images of the mice were detected on days 12 and 14. (C) Quantification of bioluminescent signals. The bioluminescence associated with MSC-Ad.GS2 and Ad.GS2 was detected and colocalized in the abdominal tumor area after intraperitoneal injection with d-luciferin. The ratio of bioluminescence is shown as the bioluminescent signal on day 14 divided by that on day 12. Values shown are mean ± SD (n = 3).