Fig. 1. Experimental strategy to identify M. bovis BCG genes that interact with rifampicin treatment and are required for intrinsic resistance. A MycoMar transposon library was generated in M. bovis BCG and selected on 7H11 solid media containing sub-MIC concentrations of rifampicin. The identity and abundance of mutants is revealed by TnSeq and compared between rifampicin treated and non-treated pools of mutants.