Table 2. Mycobacterium bovis BCG mutants with significantly altered survival upon exposure to rifampicin at 8 ng ml⁻¹ on Middlebrook 7H11 agar (Log2FC>1 or <-1, adjusted P value<0.05).

Gene locus	Gene name	Mtb H37Rv ortholog	Description	Log 2 FC	Adj P value
BCG_1782	pknE	Rv1743	Putative transmembrane serine/threonine-protein kinase E. Signal transduction	−2.69	<0.001
BCG_3073 c	–	Rv3049c	Putative monooxygenase	−2.11	<0.001
BCG_1456	–	Rv1395	Transcriptional regulatory protein for Cyp132	−2.04	<0.001
BCG_3328 c	atsB	Rv3299c	Putative arylsulfatase	−2.01	<0.001
BCG_0021	cwlM	Rv3915	Peptidoglycan synthesis regulator	−1.85	<0.001
BCG_3797	thrE	Rv3737	Putative transporter	−1.81	<0.001
BCG_1041	–	Rv0986	Putative adhesion component atp-binding protein ABC transporter	−1.62	<0.001
BCG_0380	iniB	Rv0341	Isoniazid inductible gene protein	−1.58	<0.001
BCG_0023 c	parB2	Rv3917c	Putative chromosome partitioning protein	−1.54	<0.001
BCG_1274 c	PE14	Rv1214c	PE family protein	−1.53	<0.001
BCG_2260	fabD	Rv2243	Malonyl CoA-acyl carrier protein transacylase	−1.53	<0.001
BCG_2151 c	–	Rv2134c	Hypothetical protein	−1.42	<0.001
BCG_2246 c	rnhC	Rv2228c	Bifunctional RNase H/acid phosphatase	−1.41	<0.001
BCG_1676	uvrA	Rv1638	Excinuclease ABC subunit A, DNA repair	−1.39	<0.001
BCG_0201 c	mce1R	Rv0165c	Mce1R transcriptional regulator, transport	−1.37	<0.001
BCG_3684 c	–	Rv3626c	Hypothetical protein	−1.33	0.036
BCG_1246 c	chp2	Rv1184c	Chp2 polyacyltrehalose synthesis	−1.32	<0.001
BCG_2781 c	thyA	Rv2764c	Thymidylate synthase	−1.26	<0.001
BCG_0018	rsmA	Rv3912	RsmA anti- SigM, transcription regulator	−1.21	0.046
BCG_0200	TB18.5	Rv0164	Predicted outer membrance protein	−1.18	0.033
BCG_1581	fadD24	Rv1529	Acyl-CoA synthetase	−1.16	0.049
BCG_0485 c	–	Rv0446c	Putative transmembrane protein	−1.02	<0.001
BCG_3331 c	glpD2_1	Rv3302c	Putative glycerol-3-phosphate dehydrogenase	−1.02	0.021
BCG_1863	–	Rv1828	Transcriptional regulator	−1.01	<0.001
BCG_0509	umaA1	Rv0469	Putative mycolic acid synthase	1.33	<0.001