Fig 3.

Antigen-specific memory B-cell sorting with a heterologous orthomarburgvirus GP probe. (A) Sequence divergence of the heterologous RAVV GPΔMuc B-cell sorting probe from autologous MARV Musoke and Angola immunogens. (B) Heterologous RAVV GPΔMuc probe competition for NHP1 day 49 serum neutralizing antibodies, against MARV-MLV pseudoviruses. Pre, preimmune. Shown are single replicates of a representative experiment repeated two times. (C) Memory B-cell sorting pipeline for RAVV GPΔMuc double-positive B cells with the phenotype CD27+, IgG+, CD20+, Aqua Dead-, CD3-, CD8-, CD14-, and IgM-.