Figure 2.

Monocyte-derived dendritic cells (mdDCs) and inflammatory cytokines in relation to blood TLR2 levels. mdDCs were generated from isolated monocytes (anti-CD14-coated magnetic beads) and then incubated with IL-4 and GM-CSF for 4 days. Immature mdDCs (1 × 10⁴/well) were either left untreated or stimulated with Pam3CSK4 in 96-well plates. After 48 hr, cells were incubated with 20% human AB serum in PBS containing 0.1% BSA and 0.1% NaN3 for 20 min at 4°C. After that, cells were stained with fluorescence-labeled mAb specific to CD80, CD83, CD86, and patients with elevated TLR2 values were compared with patients with normal TLR2 values on their mdDC production. There was no difference in the maturation of CD80 or CD 83 cells of mdDCs (data not shown). In TLR2-elevated patients, after stimulation with TLR2 ligand Pam3CSK4, there was significantly elevated production of TNF-α and IL-8 (a and d) and no difference in IL-1b, IL-2, IL-12p70, and IFN-α (b, c, e, and f).