Fig. 3. Comparison of immunization with Mosaic or Quartet Nanocages.
a, Schematic of antigens for this set of immunizations. b, Validation of the Alternate Quartet by SDS–PAGE with Coomassie staining, shown ±PNGase F deglycosylation. A representative gel from two independent experiments. Molecular weight markers are in kDa. c, DLS of SpyCatcher003-mi3 alone (uncoupled nanocage) or each immunogen. The mean hydrodynamic radius (RH) is shown ±1 s.d., derived from 20 scans of the sample. Uncoupled Nanocage is shown in black, with the other particles coloured as in the table inset. d, ELISA for post-boost serum IgG as the AUC of serial dilution, from mice immunized with Homotypic SARS2 Nanocages (dark blue), Mosaic-4 (green), Mosaic-8 (light blue), SpyTag-Quartet Nanocage (pink), Dual Quartet Nanocage (purple) or Uncoupled Quartet (grey). Squares indicate ELISA against a component of that vaccine (matched) while crosses indicate ELISA against an antigen absent in that vaccine (mismatched). Responses are shown to the set of sarbecovirus RBDs, with SpyTag-MBP as a negative control. The mean is shown ±1 s.d.; n = 6. Individual data points and statistics are shown in Supplementary Figs. 10 and 11.