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. 2024 Aug 17;43:229. doi: 10.1186/s13046-024-03155-z

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — The expression of CHK1, CHK2, PLK1, Aurora A, P53, pCDC25CSer216, and pCDC25CSer198 in HEY and BT-549 control cells and PGCCs with budding daughter cells with and without CDC25C knockdown. a Western blot showed the total protein expression of CHK1, CHK2, PLK1, Aurora A, P53, pCDC25CSer216, and pCDC25CSer198 in HEY and BT-549 control and PGCCs with daughter cells. b The levels of total protein expression of CHK1, CHK2, PLK1, and Aurora A in HEY and BT-549 control and PGCCs with daughter cells, which were transfected with CDC25Ci, siRNA control, and negative control. c Cytoplasmic and nuclear protein expression of CHK1, CHK2, PLK1, Aurora A, P53 in HEY and BT-549 control and PGCCs with daughter cells. d Cytoplasmic and nuclear expression of CHK1, CHK2, PLK1, and Aurora A in HEY and BT-549 control and PGCCs with daughter cells, which were transfected with CDC25Ci, siRNA control, and negative control. e The cytoplasm and nuclear protein expression of pCDC25CSer216 and pCDC25CSer198 in HEY and BT-549 control and PGCCs with daughter cells. Treatment: Cells treated with CoCl2. 1531si: siRNA CDC25C-1531