Figure 2.

IRI causes myocardial tissue damage and PMN infiltration. Graft or control hearts were harvested at POD3 from isogeneic transplants, fixed in formalin, sectioned, and H&E stained. Tissue sections were then examined and graded for severity of myocardial injury, percent of examined area showing myocardial injury, and PMN infiltrate counts. (a) Representative images of control and I/R injured cardiac tissue at POD3 with or without prior RCMV infection. Control tissue was obtained from a non-transplanted animal. Scale bars represent 100μm. (b) Myocardial injury scores on a scale of 0 (no damage) to 4 (severe damage) as described in Supplemental Table 1, (c) Myocardial injury area determined as percent of examined area showing any degree of myocardial injury, (d) PMN infiltrate as measured by number of PMN cells per field of view at 400x magnification. n=4 for all groups. Error bars represent SEM.