Figure 6.
Anakinra reduces pro-inflammatory cytokines elevated during transplantation. Luminex cytokine profiling of serum and heart tissue homogenates was analyzed following IL-1R antagonist treatment of recipients compared to non-treated controls and non-transplanted controls. Treatment groups are as indicated with (Transplant-/Treatment-) Native heart from a non-transplanted, uninfected animal (n=5); (Transplant+/VA) Graft heart from RCMV- donor with anakinra vehicle injection of recipient animals 1-hour post-transplant (n=4); (Transplant+/AD) Graft heart from RCMV- donor with anakinra treatment of donor organ pre-transplantation (n=4); (Transplant+/AR) Graft heart from RCMV- donor with anakinra treatment of recipient 1-hour post-transplant (n=4). Error bars represent SEM. Statistical significance determined by one-way ANOVA with Dunnett’s correction for multiple comparisons, *P<0.05, ***P<0.001.