Table 3.
Pro-inflammatory signaling molecules are up-regulated in graft and PBMC data following transplantation. Selected RNAseq data showing graft hearts from transplant recipients (cohort 2) versus non-transplanted native hearts (cohort 1) and PBMC samples from cohort 2 versus cohort 1. Up-regulated genes are color coded in red, down-regulated genes are color coded in blue. Those values that were found to be statistically significant (FDR-p <0.05) are bolded. Functions of each signaling molecule are listed.
| Gene | Heart: Cohort 2 Graft/Cohort 1 | FDR p- value | PBMC: Cohort 2/Cohort 1 | FDR p- value | Function |
|---|---|---|---|---|---|
| IL-1α | 1.66 | 0.4812 | Cytokine, Inflammation | ||
| IL-1β | 19.57 | 0.0000 | 3.63 | 0.4305 | Cytokine, Inflammation |
| IL1rn/IL1ra | 127.41 | 0.0000 | 4.06 | 0.0845 | Cytokine, Inhibitor |
| IL-18 | 29.50 | 0.0000 | 2.11 | 0.6595 | Cytokine, Inflammation |
| IL-18bp | 2.18 | 0.0014 | 1.76 | 0.6741 | Cytokine, Inhibitor |
| IL36β | 95.31 | 0.0000 | 3.48 | 0.0739 | Cytokine, Inflammation |
| IL33 | 3.40 | 0.0000 | 1.17 | 0.9715 | Cytokine, Inflammation |
| IL1R1 | 1.87 | 0.0181 | 12.32 | 0.0503 | Receptor, Inflammation |
| IL1R2 | 41.12 | 0.0000 | 21.46 | 0.1209 | Decoy Receptor, Inhibitor |
| IL1RAP | 1.88 | 0.0028 | 2.36 | 0.1481 | Receptor, Inflammation |
| IL18r1 | 1.69 | 0.0233 | 0.86 | 0.9050 | Receptor, Inflammation |
| IL18Rβ | 2.56 | 0.0000 | 1.95 | 0.4543 | Receptor, Inflammation |
| IL1R8 | 0.56 | 0.0014 | 0.73 | 0.6399 | Receptor, Inhibitor |
| IL1rl1 | 23.25 | 0.0000 | 2.32 | 0.5777 | Receptor, Inflammation |
| IL1rl2 | 4.86 | 0.0001 | 2.49 | 0.4433 | Receptor, Inflammation |
| Myd88 | 5.19 | 0.0000 | 1.84 | 0.0755 | Signaling Adaptor, Inflammation |
| IRAK1 | 1.29 | 0.0000 | 1.20 | 0.5414 | Signaling Adaptor, Inflammation |
| IRAK2 | 2.81 | 0.0000 | 1.24 | 0.7535 | Signaling Adaptor, Inflammation |
| IRAK4 | 4.47 | 0.0000 | 1.64 | 0.0728 | Signaling Adaptor, Inflammation |
| TRAF6 | 1.65 | 0.0006 | 1.26 | 0.8759 | Signaling Adaptor, Inflammation |
| Tollip | 0.80 | 0.0010 | 1.32 | 0.5129 | Signaling Adaptor, Inhibitor |
| Pannexin-1 | 2.32 | 0.0018 | 0.46 | 0.3481 | ATP Release Channel |
| P2X2R | 6.16 | 0.0001 | DAMP, ATP Sensor | ||
| TLR2 | 22.85 | 0.0000 | 3.39 | 0.1182 | PRR, Inflammation |
| TLR3 | 1.87 | 0.0007 | 1.01 | 0.9981 | PRR, Inflammation |
| TLR4 | 4.95 | 0.0000 | 2.00 | 0.5758 | PRR, Inflammation |
| TLR5 | 4.63 | 0.0005 | 7.24 | 0.0170 | PRR, Inflammation |
| TLR6 | 6.51 | 0.0000 | 2.17 | 0.1580 | PRR, Inflammation |
| TLR7 | 22.94 | 0.0000 | 2.25 | 0.3191 | PRR, Inflammation |
| TLR8 | 26.43 | 0.0007 | 2.22 | 0.0443 | PRR, Inflammation |
| TLR9 | 11.48 | 0.0240 | 0.76 | 0.7868 | PRR, Inflammation |
| TLR10 | 20.00 | 0.0000 | 2.24 | 0.2805 | PRR, Inflammation |
| TLR11 | 3.08 | 0.0099 | PRR, Inflammation | ||
| TLR12 | 3.83 | 0.0015 | 0.40 | 0.6166 | PRR, Inflammation |
| TLR13 | 6.92 | 0.0000 | 2.76 | 0.0691 | PRR, Inflammation |
| NLRP3 | 12.35 | 0.0000 | 7.79 | 0.1089 | Sensor, Inflammasome Formation |
| ASC | 3.75 | 0.0000 | 1.15 | 0.8267 | Adaptor, Inflammasome Formation |
| Caspase-1 | 2.75 | 0.0000 | 0.81 | 0.6099 | Enzyme, Inflammasome |
| Gasdermin D | 4.21 | 0.0000 | 0.95 | 0.9080 | Membrane Pore, Cytokine Release |
Cohort 1 = Non-transplanted, non-infected
Cohort 2 = Syngeneic transplant, non-infected
p < 0.05 is significant