Fig. 2. CircSNX5 suppression compresses cancer-associated phenotypes.

A–E CircSNX5 knockdown of oral cancer lines SAS and SCC25 were conducted (shcSNX5#1 and shcSNX5#2), and knockdown efficacy in SAS cells was evaluated by RT-qPCR assay (A). Cell proliferation (B, D) and clonogenicity (C, E) of the cells were assessed by MTT method and crystal violet staining, respectively. Colony images and quantified results of indicated cells were shown. F The mouse xenograft experiment was performed by inoculating control and circSNX5 knockdown cells. Tumors formed at the indicated time points were dissected and measured for the volume in the left panel. The photographs of mice bearing tumors and the weight of resected tumors were showed. G The wound healing migration assay of SAS cells were performed, and representative images of the indicated time points after scratching were showed. H, I Transwell migration and Matrigel invasion assays of control and knockdown SAS cells were performed. The representative photographs for indicated groups were shown in left panel, and the relative migration and invasion area were quantified and showed in bar graphs. J SAS cells were treated with doxorubicin to induce apoptosis, and circSNX5 RNA expression and PARP protein expression/cleavage of treated cells were analyzed by RT-qPCR and Western blot assays respectively. GAPDH expression served as the loading control of immunoblotting. Cell survival of treated cells were determined by MTT method.