Skip to main content
. Author manuscript; available in PMC: 2025 Jul 3.
Published in final edited form as: Methods Enzymol. 2024 Jul 3;702:1–19. doi: 10.1016/bs.mie.2024.06.012

Figure 2.

Figure 2.

Initial Rate plots for IucA, varying concentrations of one substrate at fixed concentrations of the remaining two substrates at a constant ratio relative to their respective KM values. A. Citrate concentration was varied at multiple concentrations of ATP and ahLys. B. ATP concentration was varied at multiple concentrations of citrate and ahLys. C. ahLys concentration was varied at multiple concentrations of ATP and citrate. Double reciprocal plots were created illustrating intersecting patterns for all three substrates. Reprinted with permission from Mydy, L. S., D. C. Bailey, K. D. Patel, M. R. Rice and A. M. Gulick (2020). “The Siderophore Synthetase IucA of the Aerobactin Biosynthetic Pathway Uses an Ordered Mechanism.” Biochemistry 59: 2143–2153. Copyright 2020 American Chemical Society.