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. Author manuscript; available in PMC: 2025 Jul 3.
Published in final edited form as: Methods Enzymol. 2024 Jul 3;702:1–19. doi: 10.1016/bs.mie.2024.06.012

Table 2.

Graphical analysis of kinetic data for ter-reactant enzymesa

Initial Rate Plotsb Slope and Intercept Replotsc
Mechanism 1/A 1/B 1/C ASlope AInt BSlope BInt CSlope CInt
1. Random I I I N N N N N N
2. Ordered I I I N N N L N N
3. Partially Random (AC Random) I I I N N N0 L N N
4. Partially Random (AB Random) I I 0 N0 N N0 N N *
5. Partially Random (BC Random) I I I N0 N N L N L
6. Hexa Uni Ping Pong P P P L L L L L L
7. Ordered Bi Uni Uni Bi Ping Pong I I P L L L L L N
8. Ordered Uni Uni Bi Bi Ping Pong P I I L N L L L L
9. Random Bi Uni Uni Bi Ping Pong I I P L L L L L N
10. Random Uni Uni Bi Bi Ping Pong P I I L N L L L L
a

Reprinted with permission from Mydy, L. S., Bailey, D. C., Patel, K. D., Rice, M. R. and Gulick, A. M. (2020). “The Siderophore Synthetase IucA of the Aerobactin Biosynthetic Pathway Uses an Ordered Mechanism.” Biochemistry 59: 2143–2153. Copyright 2020 American Chemical Society.

b

I, lines intersect to the left of the 1/v axis. 0, lines intersect on the 1/v axis. P, parallel lines

c

N, non-linear. L, linear. N0, non-linear, passes through the origin.

*,

all intercepts are the same.