Table 1. Sequence of primers, crRNA, and Reporter used in this study.
| Name | Sequence (5’→ 3’) |
|---|---|
| BVDV-1b primer | Forward: GCCATGCCCTTAGTAGGACT |
| Reverse: (T7 promoter sequence)/CGAACCACTGACGACTACCC | |
| crRNA 1 | (DR seq)/CAGUGGUGAGUUCGUUGGAUGGC |
| crRNA 2 | (DR seq)/ACAGUGGUGAGUUCGUUGGAUGGp |
| crRNA 3 | (DR seq)/AGUGGUGAGUUCGUUGGAUGGCU |
| crRNA 4 | (DR seq)/GGGGUAGCAACAGUGGUGAGUUC |
| DR seq | GGGGAUUUAGACUACCCCAAAAACGAAGGGGACUAAAAC |
| RNA reporter | FAM/ UUUUUUUUUUU /Biotin |
BVDV, bovine viral diarrhea virus; crRNA, clustered regularly interspaced short palindromic repeats RNA; DR seq, direct repeat.