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. 2024 Jul 31;66(4):781–791. doi: 10.5187/jast.2023.e77

Table 1. Sequence of primers, crRNA, and Reporter used in this study.

Name Sequence (5’→ 3’)
BVDV-1b primer Forward: GCCATGCCCTTAGTAGGACT
Reverse: (T7 promoter sequence)/CGAACCACTGACGACTACCC
crRNA 1 (DR seq)/CAGUGGUGAGUUCGUUGGAUGGC
crRNA 2 (DR seq)/ACAGUGGUGAGUUCGUUGGAUGGp
crRNA 3 (DR seq)/AGUGGUGAGUUCGUUGGAUGGCU
crRNA 4 (DR seq)/GGGGUAGCAACAGUGGUGAGUUC
DR seq GGGGAUUUAGACUACCCCAAAAACGAAGGGGACUAAAAC
RNA reporter FAM/ UUUUUUUUUUU /Biotin

BVDV, bovine viral diarrhea virus; crRNA, clustered regularly interspaced short palindromic repeats RNA; DR seq, direct repeat.