Skip to main content
. 2024 Jul 31;66(4):726–739. doi: 10.5187/jast.2023.e68

Fig. 3. Effect of LPA on ROS level, mitochondrial membrane potential and apoptosis in porcine SCNT-derived blastocysts.

Fig. 3.

(A) ROS levels in porcine SCNT-derived embryos exposed to LPA treatment. Experiment was conducted at least thrice, and a total of 41 (Control) and 47 (LPA group) embryos were used for the assay. (B) and (C) Fluorescence pixel ratio (red/green) and representative images of JC-1 staining (red, J-aggregates; green, J-monomers) in porcine SCNT embryos. Experiment was conducted at least thrice, and a total of 18 (Control) and 26 (LPA group) embryos were used for the assay. (D) Images of apoptotic cells in porcine SCNT blastocysts evaluated by TUNEL assays. Red indicates apoptotic cells and blue indicates nuclei. (E) Apoptosis rates (TUNEL-positive cell number/total cell number) in porcine SCNT-derived blastocysts. Experiment was conducted at least thrice, and a total of 21 (Control) and 24 (LPA group) embryos were used for the assay. (F) The expression levels of apoptosis-related genes (BAX, CASP3 and BCL2L1) in SCNT blastocysts. Different letters indicate a significant difference (p < 0.05). Scale bars = 100 μm in (C) and (D). LPA, lysophosphatidic acid; SCNT, somatic cell nuclear transfer; ROS, reactive oxygen species.