Table 4 - . Primer sequences used in the RT-qPCR assays, predicted product lengths and validation results. F: foward and R: reverse.
| Gene | Primer sequences (5’ to 3’) | Product size (bp¹) | Reaction efficiency | Linear dynamic range | r2 |
|---|---|---|---|---|---|
| Hco-pgp-2 | F: AGGATGGTGTCACGAAGAAAAT | 172 | 100% | 26.91-34.89 | 0.965 |
| R: GCCATCACAGTGCTTTTTCC | |||||
| Hco-pgp-3 | F: TCAAAGTCGTGCAGATCGAG | 107 | 99% | 23.05-32.50 | 0.993 |
| R: AGTTGTCACGAGCACTTTCA | |||||
| Hco-pgp-9a | F: ACGTGAAGTGAACCCAACTC | 197 | 91% | 24.13-32.16 | 0.989 |
| R: TGTTGTAACCATCTGGCAGG | |||||
| Hco-pgp-10 | F: CAGAAAGATTATGCGCCACG | 199 | 104% | 26.55-31.94 | 0.954 |
| R: GAGTGCCATCTTCCAGTTGA | |||||
| Hco-pgp-11 | F: GTTTTGGTGGATGGTCAGGT | 166 | 95% | 22.63-32.78 | 0.996 |
| R: TCGAGACACGCTTGAACATC | |||||
| Hco-pgp-16 | F: TTGAATCCTTGAACACCGCG | 164 | 91% | 23.32-30.92 | 0.994 |
| R: TCCGCGTAACTTAGTTTGCA | |||||
| Hco-haf-9 | F: CAGGTCTGTCACGGAAAACA | 170 | 94% | 20.78-30.67 | 0.991 |
| R: GTTGCTTTTGTCCACCTGAC | |||||
| Hco-gapdh | F: GAGCACTCACAGGATCAAGG | 163 | 95% | 15.40-25.32 | 0.993 |
| R: AGCAGAGATGACGACCTTCT | |||||
| Hco-act | F: GCTCCCAGCACGATGAAAA | 100 | 96% | 18.20-28.06 | 0.985 |
| R: CACCAATCCAGACAGAGTATTTGC | |||||
| Hco-prl9 | F: TCAAGGGTGTCACTAAGGGT | 115 | 91% | 16.20-26.22 | 0.985 |
| R: CCGAGGAAGTTACGAATCTCA | |||||
| Hco-ftt-2 | F: CGATTGAGCAGAAGACGGAA | 113 | 98% | 14.24-23.99 | 0.995 |
| R: GAGCAGGTTCAAAACGTCCT |
¹
base pairs