Fig. 1.

HBx integration in DLBCL.

(A) Five clinical tissue samples of DLBCL from serum HBsAg positive patients (Number 1 to 3) and negative patients (Number 4 and 5) were produced following nucleic acid extraction. HBx gene (HBx) was detected using the PCR method, and a Genome Walking analysis of integration (GW3) was performed. (B) HBx plasmid was electrotransferred into DLBCL cells, Pfeiffer strain. Genome walking was used to verify the integration of the HBx gene into the genome of clonal cells. (C) WGS analysis of HBx transfected DLBCLs performed using the next-generation sequencing platform (Illumina HiSeq). The left panel shows the breakpoint location of HBx integration found in the human genome. The right panel shows the distribution of cytoplasmic reads during sequencing and the breakpoint location of the plasmid during HBx integration.