Fig. 5.

HBx integrated into clinical DLBCL tissues and was associated with apoptosis marker.

(A) HBx molecules were detected using FISH method in the DLBCL tissue chip (HLymB085PT01), and the integration of HBx genes in situ was demonstrated using specific Cy3 probe-red. The HBx positive (n = 26, 37.1 %) and negative (n = 44, 62.9 %) groups were stratified based on the results of FISH test. (B) The tumor tissue chip was fixed, and the expression of cleaved PARP was detected using IHC method. The IRS between HBx integration and negative group was compared in (C). And the framed spots labelled with coordinates in (A) and (B) were enlarged and displayed in (D) and (E) respectively. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)