TABLE 3.

Effects of different concentrations of rutin (R) and chlorogenic acid (CGA) on buffalo sperm total and progressive at the post‐thaw stage of cryopreservation.

Analysis	Control	R‐0.4	R‐0.6	R‐0.8	R‐1	CGA‐50	CGA‐100	CGA‐150	CGA‐200
Total motility (%)	54.03 ± 1.54fg	61.05 ± 1.20cd	65.18 ± 1.36ab	57.32 ± 1.42ef	59.03 ± 1.19de	61.49 ± 1.73cd	64.59 ± 1.51bc	68.24 ± 1.14a	53.72 ± 1.30g
Progressive motility (%)	22.19 ± 1.40f	30.82 ± 1.89cd	34.03 ± 1.65ab	25.92 ± 1.15ef	27.45 ± 1.37de	30.35 ± 1.63cd	33.54 ± 1.90bc	37.89 ± 1.78a	23.45 ± 1.20f

Note: Values are expressed as mean ± SEM. R‐0.4: Tris‐based extender + rutin (0.4 mM); R‐0.6: Tris‐based extender + rutin (0.6 mM); R‐0.8: Tris‐based extender + rutin (0.8 mM); R‐1: Tris‐based extender + rutin (1 mM); CGA‐50: Tris‐based extender + chlorogenic acid (50 µM); CGA‐100: Tris‐based extender + chlorogenic acid (100 µM); CGA‐150: Tris‐based extender + chlorogenic acid (150 µM); CGA‐200: Tris‐based extender + chlorogenic acid (200 µM). Different superscripts within the same row demonstrate significant differences (p ≤ 0.05).