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. 2024 Aug 2;28(4):474. doi: 10.3892/ol.2024.14607

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Copyright: © 2024 Chang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — Effects of CANX and DHA on mitochondrial ATP production and the NAD⁺/NADH ratio. (A) ATP fluorescence intensity probe detected ATP fluorescence intensity of HuH-7 and Li-7 cells after CANX knockout, DHA or CANX overexpression. Level of ATP fluorescence intensity in (B) HuH-7 and (C) Li-7 cells, quantified using Image-Pro Plus 6.0 software. Ratio of NAD⁺ and NADH content in (D) HuH-7 and (E) Li-7 cells after CANX knockout, DHA or CANX overexpression (scale bar, 20 µm), **P<0.01; ***P<0.001. CANX, calnexin; DHA, dihydroartemisinin; NC, negative control; siRNA, small interfering RNA.