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. Author manuscript; available in PMC: 2024 Aug 19.
Published in final edited form as: Nature. 2023 Jul 26;621(7977):154–161. doi: 10.1038/s41586-023-06456-z

Fig. 1 |. Apo monomeric MapSPARTA.

Fig. 1 |

a, Domain architecture of TIR-APAZ and pAgo that together form MapSPARTA. The TIR domain is indicated in green, APZA blue, MID in magenta, and PIWI in yellow.

b, c, Cryo-EM density map (b) and ribbon diagrams (c) of apo monomeric MapSPARTA with domains colored as in a.

d, Surface representation of apo monomeric MapSPARTA with the nucleic acid-binding pocket indicated with yellow dashed lines and the auto-inhibitory C-terminal motif (CTM) of APAZ shown in red.

e, Detailed interactions between APAZ CTM and pAgo.

f, g, BLI sensorgrams of wildtype (f) and C-terminal motif (CTM)-truncated MapSPARTA (g) binding the target DNA. From top curve to bottom, the proteins were two-fold serial dilutions from 50 nM.