Skip to main content
. Author manuscript; available in PMC: 2024 Aug 19.
Published in final edited form as: Nature. 2023 Jul 26;621(7977):154–161. doi: 10.1038/s41586-023-06456-z

Extended Data Fig. 8 |. Interfaces between MapSPARTA and RNA/DNA duplex.

Extended Data Fig. 8 |

a,Schematic depiction of the detailed interactions between MapSPARTA and RNA/DNA duplex. Residues from PIWI domain, MID domain and APAZ domain are colored in yellow, pink, and blue, respectively.

b,Detailed interactions between PIWI domain and RNA-DNA duplex. Residues involved in coordinating RNA-DNA duplex are highlighted in sticks. DNA and RNA bases are labelled in green and red, respectively.

c, An enlarged view of the interface between APAZ and RNA-DNA duplex. Residues involved in interacting with RNA-DNA duplex are highlighted in sticks. DNA and RNA bases are labelled in green and red, respectively.

d, Overlaid structures of apo MapSPARTA and MapSPARTA with RNA/DNA, revealing the tilting of negative-charged motif and positive-charged pocket critical for MID-MID interactions mediated dimerization.